Is G418 the same as neomycin?

Is G418 the same as neomycin?

G418 is an analog of neomycin sulfate, and has similar mechanism as neomycin. G418 is commonly used in laboratory research to select genetically engineered cells .

Can I use neomycin instead of G418?

Bottom line: Use G418 for single-selection experiments on eukaryotic cells. Use neomycin or kanamycin for single-selection experiments on prokaryotic cells where you are selecting for neomycin resistance genes. Kanamycin should be used over neomycin if elimination of Mycoplasma species from culture is necessary.

How does puromycin selection work?

This compound works by interrupting protein synthesis in both prokaryotic and eukaryotic cells. This causes cells to die, unless they have a gene for resistance, allowing them to survive exposure to the antibiotic.

What is the concentration of puromycin for selection?

0.5 – 10 µg/ml
Puromycin antibiotic ensures effective positive selection of cells expressing the puromycin-N-acetyl- transferase (pac) gene. In mammalian cells, the recommended working concentration range for puromycin is 0.5 – 10 µg/ml.

How long does puromycin selection take?

Puromycin selection requires a minimum of 48 hours. Optimum effectiveness should be reached within 3-10 days. Assay transfected cells.

Which is the best concentration of G418 to use?

G418 is normally used at a concentration of 400 μg/ml. However, the optimal concentration needs to be determined for your cells. Suggested concentrations of G418 for selection in some examples of mammalian cells are listed below (with references on the next page). Cell line Medium G418 conc. Ref.

How long does it take to use G418 in cell culture?

G418 (Geneticin) : selection antibiotic, cell culture tested, sterile reagent. Selection in mammalian cells is usually achieved in 3 to 7 days with concentrations ranging from 400 to 1000 µg/ml. Cells that are dividing are affected more rapidly than non-dividing cells.

What is the working concentration of G418 in the neo gene?

The working concentration of G418 for selection and maintenance of mammalian cell lines transfected with the neo gene varies depending on a multitude of factors, including cell type.

How does G418 work as a selection antibiotic?

G418 (Geneticin) : selection antibiotic, endotoxin tested, sterile reagent G418 also known as G-418, G418 sulfate or Geneticin is an aminoglycoside antibiotic produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.