What is Qubit protein Assay?

What is Qubit protein Assay?

Description. The Qubit Protein Assay Kit is designed specifically for use with the Qubit Fluorometer. Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation.

Is RIPA buffer compatible with Qubit protein assay?

Therefore, it’s still usable even for the samples in Laemmli buffer. Although the method takes longer time than other method, I’m quite satisfied with the results. just making sure your RIPA doesn’t have any detergents in it. SDS, tween20 and tx100 all are contaminants for the qubit assay.

What does the Qubit measure?

The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA.

How does the Qubit fluorometer work?

The Qubit fluorometer uses fluorescent dyes that emit signals only when bound to the specific target molecules (DNA or RNA) even in the presence of free nucleotides, degraded nucleic acids, or protein contaminants.

How does NanoDrop measure protein concentration?

The NanoDrop One Thermo Scientific™ Acclaro™ Sample Intelligence Technology uses mathematical algorithms to detect nucleic acids in protein samples and correct the concentration result as needed. Sample purity can also be assessed by looking at the A260/A280 value.

How is the qubit protein assay kit used?

The Qubit Protein Assay Kit is designed specifically for use with the Qubit Fluorometer. Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation.

Can a Quant iT kit be used with a Qubit fluorometer?

In addition, the new Quant-iT kits (labeled as “for use with the Qubit Fluorometer”) have standards formulated to be compatible with the Qubit fluorometer internal algorithms for the respective assays. The Qubit Fluorometer-compatible kits are also less expensive per assay if you are processing fewer than 20 samples at a time.

How big of a sample do you need for qubit?

Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation. The assay is highly selective for proteins and is designed to be accurate in the presence of reducing reagents, but not in the presence of a large amount of detergent.

Can a Qubit DNA and RNA kit be used?

No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument.