What is staining in cytology?
What is staining in cytology?
To perform a cytology staining, stains reveal the structures of the cells to examine such as the nucleus, the cytoplasm, and cellular granules. Experience is necessary to obtain an optimal smear, a fine balance between too thick and too thin smears. After preparing the smears, fixation, and staining is essential.
What is the principle of Giemsa stain?
PRINCIPLE: The “neutral” dyes combining the basic dye methylene blue and the acid dye eosin, give a wide color range when staining. The pH of the staining solution is critical and ideally should be adjusted for different fixatives.
What is cytology fixative used for?
Cytology Fixative covers cells with a tough, soluble film that protects cell morphology for microscopic examination. It is water and alcohol soluble, environmentally friendly and extremely economical.
What is a cytology sample?
What is cytology? Cytology is the exam of a single cell type, as often found in fluid specimens. It’s mainly used to diagnose or screen for cancer. It’s also used to screen for fetal abnormalities, for pap smears, to diagnose infectious organisms, and in other screening and diagnostic areas.
What stains are used in cytology?
The universal stain for cytological preparations is the Papanicolaou stain. Harris’ hematoxylin is the optimum nuclear stain and the combination of OG6 and EA50 give the subtle range of green, blue and pink hues to the cell cytoplasm.
How do you stain a cytology?
Here are some tips for this procedure:
- Make sure your sample on the slide is dry.
- Stain only one slide. Always submit 2-4 unstained slides with the stained slide.
- Don’t stain the best slide.
- Stain cytology slides in uncontaminated jars. Separate these jars from the stain used for infected samples, such as ear cytology.
Why is Giemsa stain used?
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
Is Giemsa acidic or basic?
The polychromatic staining solutions (Giemsa, Wright) contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. Methanol acts as fixative and also as solvent. The fixative does not allow any further change in the cells and makes them adhere to the glass slide.
How is the Giemsa stain used in cytogenetics?
Giemsa stain also is used to stain Histoplasma capsulatum, Pneumocystis jiroveci, Klebsiella granulomatis, Penicillium marneffei and occasionally bacterial capsules. This stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations.
How long does it take to dilute a Giemsa stain?
Stain with diluted Giemsa stain (1:20, vol/vol) for 20 min (For a 1:20 dilution, add 2 ml of stock Giemsa to 40 ml of buffered water in a Coplin jar). Wash by briefly dipping the slide in and out of a Coplin jar of buffered water (one or two dips).
Can you use methanol as a fixative in Giemsa stain?
Methanol act as a fixative as well as the cellular stain. The fixative does not allow any further change in the cells and makes them adhere to the glass slide. Giemsa stain can be prepared in house using Giemsa stain powder or can be commercially obtained.
Which is the best stain to use in cytology?
The universal stain for cytological preparations is the Papanicolaou stain.Harris’ hematoxylin is the optimum nuclear stain May-Grünwald Giemsa Stain is one of the common Romanwsky stains usedin cytology. It is useful for studying cell morphology in air-dried smears.