What is OT-1 mice?
What is OT-1 mice?
Also Known As:OT-1 These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. These mice are often used to study CD8+ T cell response to antigen, positive selection, and in any research requiring CD8+ T cells of defined specificity. Like most TCR transgenics, these mice are somewhat immunodeficient.
What are ot i cells?
These SIINFEKL-specific CD8+ T cells, commonly referred to as ‘OT-I T cells’, have a T cell antigen receptor that consists of α-chain variable region 2 (Vα2) and β-chain variable region 5 (Vβ5), which are inherited via a single transgene1.
Do OT-1 mice have CD4 T cells?
Most dramatically, ≈90% of Vα2+ T cells in OT-1+RAG+TAP−/− mice were CD4+ (Fig. 3B). The OT-1 TCRβ chain has been shown to pair with TCRα chains that differ only in the CDR3 region to generate both CD4+ and CD8+ T cells in mice (36, 37).
What is Oti mouse?
OT I Mouse Details These homozygous mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin residues 257-264 in the context of H2Kb and used to study the role of peptides in positive selection and the response of CD8+ T cells to antigen.
What is Siinfekl?
SIINFEKL genetically incorporated into viral or cellular source proteins can be used to precisely probe various aspects of antigen presentation, including the kinetics of peptide generation, MHC class I surface stability, and presentation efficiency following pharmacological and genetic manipulations including genome …
Why is ovalbumin used in immunology?
Chicken OVA is a T Cell-dependent antigen commonly used as a model protein for studying antigen-specific immune responses in mice. It is non-toxic and inert and therefore will not cause harm to the animals even if no immune response is induced.
What is TCR transgenic mice?
To overcome this limitation, transgenic (Tg) mice with high frequencies of particular TCRs are generated. These TCR Tg mice allow researchers to efficiently study T cell-mediated immune responses according to antigen-specific TCR activity by synchronizing all T cell responses to one epitope [4].
Where is ovalbumin found?
egg white
Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up approximately 55% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor.
What is OVA peptide?
OVA Peptide is a class I (Kb)-restricted peptide epitope of ovalbumin presented by the class I MHC (major histocompatibility complex) molecule, H-2Kb (class I genes of the mouse MHC).
What kind of protein is ovalbumin?
egg white protein
Ovalbumin is the predominant protein in albumen and represents 54% to 58% of the egg white protein by weight. It is a monomeric phosphoglyco-protein with a molecular weight of 44.5 kDa and an isoelectric point (IEP) of 4.5.
What is Siinfekl peptide?
Ovalbumin peptide SIINFEKL (H-2 Kb) is a single peptide for stimulation of T cells. The peptide from Ovalbumin is synthesized as it is presented by the MHC class I H-2 Kb allele and can be used for targeted in vitro generation and expansion of antigen-specific CD8+ T cells.
How are TCR transgenic mice made?
The production of TCR Tg mice starts by generating genomic constructs for transformation. Genes encoding TCR α- or β-chains are extracted from T cell clones expressing antigen-specific TCRs. T cell clones are generated through several methods [10,11] that are followed by the selection of a specific T cell clone.